278 PROTEIN POISONS 



9. The poison formed by the action of the ferment on 

 the protein passes through hardened filter paper. One 

 milligram of egg-white protein was added to each 5 c.c. 

 of serum and organ extracts obtained from a guinea-pig 

 killed twenty days after sensitization to egg-white; then 

 these portions were incubated for thirty minutes, filtered 

 through hardened paper, and injected intracardiacly in 

 fresh guinea-pigs, with the results shown in Table XXXII. 



TABLE XXXII 



No. Fluid. Effect. 



1 .... Serum Convulsions, recovery 



2 .... Liver Dead in 12 minutes 



3 .... Kidney Dead in 2 minutes 



4 ... Spleen First and second stages 



10. The poison passes through a Berkefeld V. The 

 serum and organ extracts of a guinea-pig, killed twenty- 

 three days after sensitization to egg-white, were treated 

 with 1 mg. of egg-white protein to each 5 c.c., incubated 

 for thirty minutes, filtered through a Berkefeld V, and 

 the filtrates injected intracardiacly in fresh guinea-pigs, 

 with the results shown in Table XXXIII. 



TABLE XXXIII 

 No. Fluid. Effect. 



1 .... Serum (3 c.c.) First and second stages 



2 .... Liver Dead in 6 minutes 



3 .... Spleen Dead in 10 minutes 



4 .... Kidney Dead in 9 minutes 



11. Serum and organ extracts from sensitized animals 

 are inactivated when heated to 56 for thirty minutes. 



In our first experiment on this point the fluids were 

 placed in small Erlenmeyer flasks, and these were set in 

 water at 56 and allowed to stand for thirty minutes; then 

 5 c.c. portions, to each of which 1 mg. of egg-white protein 

 was added, were kept in the incubator for thirty minutes. 

 The result was that the inactivation was incomplete, as 

 shown by Table XXXIV. 



