PROTEIN SENSITIZATION OR ANAPHYLAXIS 279 



TABLE XXXIV 



No. Fluid. Effect. 



1 .... Serum First and second stages 



2 .... Liver Dead in 4 minutes 



3 .... Kidney First stage 



4 .... Spleen None 



As a control to the experiments of Table XXXIV, animals 

 were treated with the serum and organ extracts from the 

 same animal to which egg-white had been added and incu- 

 bated for thirty minutes without previous subjection to 

 heat. The results are shown in Table XXXIV A. 



TABLE XXXIV A 



No. Fluid. Effect. 



1 .... Serum (3 c.c.) Dead in 6 minutes 



2 .... Liver Dead in 6 minutes 



3 .... Kidney Dead in 5 minutes 



4 .... Spleen First and second stages 



In a repetition of this test, the serum and extracts were 

 placed in thin sealed tubes and kept submerged for thirty 

 minutes in water at the temperature of 56. After this 

 the fluids were treated with 1 mg. of egg-white protein, 

 incubated for thirty minutes, and injected into the hearts 

 of normal guinea-pigs, with the results shown in Table 

 XXXIV B. 



TABLE XXXIV B 



No. Fluid. Effect 



1 .... Serum None 



2 .... Kidney None 



3 .... Liver None 



4 .... Spleen None 



As a control to the experiments of Table XXXIV B, the 

 unheated extracts from the same animal were employed, 

 with results as shown in Table XXXIV c. There was not 

 enough serum to use in the control. 



