280 PROTEIN POISONS 



TABLE XXXIV c 



No. Fluid. Effect. 



1 .... Kidney Dead in 8 minutes 



2 .... Liver Dead in 5 minutes 



3 .... Spleen Dead in 4 minutes 



12. Serum and organ extracts inactivated by heating 

 to 56 may be reactivated by the addition of corresponding 

 fluids obtained from an unsensitized animal. The serum 

 and organ extracts obtained from an animal killed twenty- 

 seven days after being sensitized to egg-white were inacti- 

 vated by being heated to 56, then treated with equal 

 volumes of serum and corresponding organ extracts, egg- 

 white added, 1 mg. to each 5 c.c. of fluid, and incubated 

 for thirty minutes. These fluids when injected into the 

 hearts of unsensitized animals produced the results shown 

 in Table XXXV. 



TABLE XXXV 



No. Fluid. Effect. 



1 .... Serum (3 c.c.) First and second stages 



2 .... Liver Dead in 34 minutes 



3 .... Kidney Dead in 56 minutes 



4 .... Spleen Dead in 45 minutes 



It will be noticed that when reactivated fluids were used, 

 death was not so speedy. 



As a control to the experiments of Table XXXV, the 

 inactivated fluids from the same animal were used without 

 the addition of complement, with results shown in Table 

 XXXV A. 



TABLE XXXV A 



No. Fluid. Effect. 



1 .... Serum (3 c.c.) None 



2 .... Liver None 



3 .... Kidney None 



4 .... Spleen None 



13. The serum or organ extracts of animals sensitized 

 to egg-white do not produce a poison when incubated with 



