304 PROTEIN POISONS 



abdominal cavity of a fresh guinea-pig of about the same 

 weight. Fourteen days later this animal received intra- 

 venously 1.5 c.c. of lamb serum, and promptly died of 

 anaphylactic shock. Autopsy showed the lungs distended, 

 the heart still beating, and the blood of the right heart had 

 not coagulated at the expiration of ten minutes. 



The organ may be implanted into another species, as is 

 shown by the following: A rabbit was treated with lamb 

 serum until the precipitation titer of the serum was 1 to 

 1000; then the rabbit was exsanguinated and perfused with 

 salt solution, and two pieces of its spleen implanted in 

 the abdominal cavity of a guinea-pig. Fourteen days 

 later this guinea-pig received intravenously 1.5 c.c. of lamb 

 serum, and died of anaphylactic shock. Guinea-pigs thus 

 treated were found to be sensitized not only to lambs, but 

 also to rabbit serum, thus proving that the implanted 

 organs continued to secrete both their normal and their 

 specifically developed proteolytic ferments (antibodies). 

 Like results were obtained when pieces of kidney were 

 transplanted. Moreover, the animals thus sensitized by 

 the receipt of transplanted organs retained their sensitized 

 condition for a time at least after the removal of the im- 

 planted tissue. The following is an illustration. A guinea- 

 pig received intravenously 1 c.c. of lamb serum. Five 

 days later it had two subcutaneous injections of 1 c.c. of 

 lamb serum. Eight days later it was killed and transfused, 

 and portions of its spleen and kidney implanted in a fresh 

 guinea-pig. Six days later the implanted tissues were 

 wholly removed, and after the guinea-pig had fully recovered 

 from this operation it was found to be still sensitized to 

 lamb serum. Evidently the implanted tissue had not only 

 continued to develop its specific ferment, but had discharged 

 it in part at least into the blood. These experiments are 

 of the highest value for two reasons: (1) They caused 

 Friedberger to wholly abandon his theory of sessile receptors, 

 and (2) they show that the specific ferment developed in 

 protein sensitization is a cellular product, and that the 

 cells of the spleen and kidney, possibly of other organs as 

 well, elaborate it. 



