PROTEIN SENSITIZATION OR ANAPHYLAXIS 305 



Later this work was continued by Girgolaff, 1 who discusses 

 the possible explanation of these findings. (1) It might 

 be possible that the recipient is passively anaphylactized 

 by the transfer of some serum from the donor. This suppo- 

 sition is held untenable for two reasons. First, the washing 

 out is so thoroughly done, and second, the volume of the 

 piece of organ transferred is too small. Moreover, if it were 

 passive anaphylaxis, the recipient should be most respon- 

 sive to reinjection within a day or two, while in fact it is 

 not responsive until after seven or eight days. (2) It might 

 be suggested that some of the protein used in sensitizing 

 the first animal is carried over to the second and actively 

 sensitizes it. This is highly improbable on account of the 

 small amount of protein used in sensitizing the first animal; 

 the length of time (in some cases fourteen days) elapsing 

 before the transfer of the tissue, and the thorough washing 

 out given the first animal. Besides, this was shown to be 

 impossible because in one instance a rabbit was killed and 

 its organs transferred to another rabbit three hours after 

 the former had received a bacterial suspension, just at the 

 time when the bacilli should have been most abundant in 

 the tissue, and these animals were not sensitized. (3) The 

 only conclusion which seems to have any justification is 

 that the cells of the tissue removed, having acquired a new 

 function while in their normal location, continue to exercise 

 this function in their new location. We regard this as a most 

 complete verification of our theory of anaphylaxis, in which 

 we hold that a new function is developed in certain cells of 

 the animal body by the sensitizer. Moreover, it does seem 

 that this work should lead to the discarding of all theories 

 involving an " antigenrest, " about which much has been 

 said. 



Vaughan, Vaughan, Jr., and Wright 2 demonstrated that 

 the serum and organ extracts of normal guinea-pigs do 

 not form a poison when incubated with egg-white, but 



1 Zeitsch. f. Immunitatsforschung, 1912, xii, 401. 



2 Ibid., 1911, xi, 673. 

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