358 PROTEIN POISONS 



drawn from the heart and injected into guinea-pigs. The 

 first two were found to be sensitized while the others were 

 not. 



5. Egg-white injected intravenously in rabbits may be 

 detected in the peritoneal cavity after it has disappeared 

 from the circulating blood. 



January 3, 1910, a rabbit received intravenously 50 c.c. 

 of the egg-white dilution. Two and one-half hours later 

 and after the egg-white had disappeared from the heart's 

 blood, as was shown by a subsequent test, some physio- 

 logical salt solution was injected into the peritoneal cavity, 

 withdrawn and injected into a guinea-pig, which later was 

 found to be sensitized to egg-white. 



6. Egg-white injected intravenously into rabbits may 

 be detected in the bile. 



November 15, 1909, a rabbit received intravenously 50 

 c.c. of the egg-white dilution, one and one-half hours later 

 the abdominal cavity was opened, the animal being under 

 ether, and small amounts of bile and washings from the 

 small intestine were injected into guinea-pigs, all of which 

 later were found to be sensitized to egg-white. Of four 

 pigs thus treated all but one died, and this one developed 

 marked symptoms when treated with the egg-white dilution. 



7. Egg-white when injected intravenously into a rabbit 

 may be detected by the sensitization test in certain organs 

 after it has disappeared from the circulating blood. 



December 6, 1909, at 8.45 A.M., a rabbit received intra- 

 venously 50 c.c. of a dilution of egg-white with an equal 

 volume of physiological salt solution. 5 c.c. of blood was 

 drawn from the heart of this animal at 9.45, 10.45, and 

 11.45 A.M., and at 1.45 P.M. Each of these portions was 

 injected into the abdomen of a guinea-pig and one hour 

 after the last blood was taken the rabbit was killed with 

 ether, and extracts of the brain, liver, kidney, and spleen, 

 with physiological salt solution were made and injected 

 into other fresh pigs. December 17, 1909, each of the pigs 

 that had been treated with the blood of the rabbit had 

 5 c.c. of egg-white dilution (1 to 1) intra-abdominally. 



