360 PROTEIN POISONS 



2 c.c. portions of the fluid were drawn from the heart and 

 injected into guinea-pigs. The last of these portions was 

 drawn after one liter of the salt solution had passed through. 

 All of these portions sensitized the guinea-pigs. After the 

 transfusion, the brain, liver, spleen, kidney, and the deltoid 

 muscle were removed, and rubbed up with physiological 

 salt solution. 2 c.c. of each of these extracts, after filtra- 

 tion, was injected into guinea-pigs. The one having the 

 brain extract was not affected by the subsequent injection 

 of egg-white. The one having the liver extract was in 

 convulsions within five minutes after receiving the egg- 

 white. The ones having the extracts from the spleen and 

 muscle developed first and second stages of sensitization, 

 but recovered, while the one that received the kidney 

 extract was not affected. 



We conclude from this that the brain and kidney were 

 washed free of the egg-white by the transfusion, while the 

 muscle, liver, and spleen held the egg-white more tena- 

 ciously. 



9. The injection of egg-white intravenously in rabbits 

 decreases after a few hours the total protein in the blood. 



In reviewing the literature we have referred to the finding 

 of Chiray that the intravenous injection of foreign proteins 

 decreases the total proteins of the blood. His experiments 

 were made with a refractometer. We deemed this of 

 sufficient importance to justify further study. On one 

 day blood was drawn from a rabbit and the serum obtained. 

 On the next day this animal received 50 c.c. of the filtered 

 egg-white dilution intravenously. Each cubic centimeter 

 of this dilution of egg-white contained 26 mg. of protein, 

 as calculated from a nitrogen determination. In other 

 words, with the dilution there was introduced into the blood 

 of the rabbit 1.3 grams of foreign protein. On the day after 

 the injection more blood was drawn and the serum from 

 this secured. The total nitrogen in these sera was deter- 

 mined and the protein content calculated with the following 

 results : 



