PROTEIN FEVER 377 



nally, into each of 5 guinea-pigs for the purpose of sensitizing 

 them to whatever proteins the urine might contain. Twelve 

 days later 2 of these animals received intra-abdominally, 

 each 5 c.c. of egg-white dilution (with an equal volume of 

 water); 2 others had, each 5 c.c. of fresh serum from a 

 rabbit, and the fifth had a mixture of 2.5 c.c. of each of 

 these fluids. All were found to be sensitized, thus showing 

 the presence of both egg-white and serum protein in the 

 urine of the febrile rabbit. 



It is worthy of note that while these animals developed 

 the three stages characteristic of protein sensitization, the 

 second and third stages were unusually prolonged and less 

 acute than those generally observed in sensitized guinea- 

 pigs. Of the two treated with egg-white, one died at the 

 end of two hours and the other fifteen minutes later. Of 

 the two treated with rabbit serum, one died at the end of 

 one hour, while the other lived for three hours. The one 

 that had the mixture of proteins developed the symptoms 

 more promptly than any of the others, but did not die. 



A continued fever was maintained in another rabbit by 

 injections of the same strength of egg-white solution from 

 April 30 to May 18, 1909. In this instance the size of the 

 dose was not altered. The animal received four doses daily 

 from April 30 to May 11, after which five were given until 

 May 15, and then for three days we returned to four doses 

 daily. The fever continued, after the injections were 

 discontinued, until the evening of May 20, when it fell by 

 crisis below the normal, slowly returning to the normal. 

 The urine was collected and nitrogen determined as in the 

 other instance, but the charts are so similar that we do 

 not consider it necessary to present the second one. 



The Production of Continued Fever in Rabbits by Repeated 

 Subcutaneous Injections of the Poisonous Group of the Typhoid 

 Protein. Fig. 13 shows the effects of repeated subcutaneous 

 injections of sublethal doses of the poisonous group split off 

 from the cellular substance of the typhoid bacillus with a 2 

 per cent, solution of sodium hydroxide in absolute alcohol. 



The material used was the crude soluble poison containing 



