PROTEIN FEVER 395 



in the article referred to, but also to the skin and walls of 

 the alimentary canal. 



We have also attempted to determine how long after 

 injection egg-white can be detected in the tissues. 



Three rabbits received intravenously 25 c.c. of egg-white 

 dilution (1 to 1). One was killed twenty-four hours later, 

 and sections of its skin, kidney, brain, liver, spleen, and 

 intestinal and stomach walls rubbed up with salt solution. 

 After standing in the cold room overnight these emulsions 

 were filtered and the filtrates injected intra-abdominally 

 into guinea-pigs. The second rabbit was killed after forty- 

 eight hours, and the third after seventy-two hours, and their 

 tissues treated in the same way. All the guinea-pigs that 

 received extracts from the first and second rabbits were 

 found to be sensitized, though none died. Choking symp- 

 toms were very marked, most pronounced in those that 

 received extracts from the spleen and kidney. The symptoms 

 were quite as marked in those that received the extracts 

 from the second rabbit as in those treated with the extracts 

 from the first. The pigs that received the extracts from the 

 third rabbit showed absolutely no symptoms. 



From these experiments we conclude that egg-white 

 diffused through the tissues after injection into the blood 

 becomes, sometime between two and three days, either 

 so far changed as to loose its identity or so fixed in the 

 tissue that it cannot be washed out with salt solution. This 

 time interval probably varies with the kind and amount 

 of foreign protein introduced, and in different species of 

 animals. 



The Digestive Action of the Blood Serum of Rabbits in Which 

 Fever Has Been Induced with Egg-white. The following 

 illustrates some of our experiments on the digestive action 

 of the blood serum: The temperature of two rabbits was 

 raised to 106 by hourly intravenous doses of a dilution 

 of egg-white (1 to 1). One hour after the last injection 

 both of these animals were bled to death from the jugular 

 vein and the serum obtained. 



Two cubic centimeters of this fever serum without any 



