SPECIFIC FERMENTS OF THE CANCER CELL 431 



filter, definite amounts were analyzed by the Scherer and 

 Hammersten methods for total albumins and globulins. 



Determination of Albumin and Globulin. 25 c.c. of leuko- 

 cyte extract which had been centrifugated to throw down 

 all corpuscles and sand used in its preparation was passed 

 through a filter paper. This was rendered acid with acetic 

 acid, 4 grams of NaCl added, and then heated for one-half 

 hour on a water-bath. After coagulation had occurred this 

 was filtered through a previously dried and weighed filter 

 and washed with hot water until the filtrate ceased to give 

 a reaction for chlorides. Next, the residue was washed 

 with absolute alcohol and then ether, after which it was 

 dried at 130 to a constant weight. 



Paper and albumin plus globulin . . . 1 . 66658 grams 

 Weight of paper 1.61 150 grams 



Weight of albumin and globulin .... 0.05508 gram 



The same method was applied to 25 c.c. of the same lot 

 of leukocyte extract after passing through a Berkefeld 

 filter. 



Weight of filter plus albumin and globulin . 1 . 55000 grams 

 Weight of filter 1.53125 grams 



Weight of albumin and globulin .... 0.01875 gram 



In order to make a separate determination of globulins 

 and albumins the following method of Hammersten was 

 adopted: 25 c.c. of leukocyte extract before filtration 

 through a Berkefeld was added to 30 grams of pulverized 

 magnesium sulphate. This was warmed to 30 with fre- 

 quent stirring, and then placed in the cold for twenty-four 

 hours. This was then filtered through a weighed filter, 

 previously dried at 110, and washed with magnesium 

 sulphate until the filtrate ceased to give a reaction for 

 albumin when heated with acetic acid. Next, the filter 

 was dried for four hours at 110 to coagulate the globulin, 

 after which the magnesium sulphate was washed out with 



