SPECIFIC FERMENTS OF THE CANCER CELL 435 



is in all probability a part of the globulin content, and its 

 activity is much increased by obtaining it in the more 

 purified state. 



It is now my practice to test the strength of each batch 

 prepared by the above experiment before using it in any 

 given case. Sensitization phenomena have been entirely 

 lacking whenever used therapeutically, which was not 

 true of any former preparations. The filtered leukocyte 

 extract when used in small amount subcutaneously rarely 

 produced sensitization phenomena, but when injected 

 intravenously in increased dosage this symptom complex 

 was frequently observed. The unfiltered leukocyte extract 

 frequently produced sensitization even with small amounts 

 given subcutaneously, so the conclusion previously arrived 

 at; that the albumin content was responsible for these 

 untoward symptoms is apparently confirmed. It must not 

 be understood, however, that the globulins do not sensitize. 

 The work so far simply shows that it is possible to remove 

 the albumin which contains no specific ferment, and inas- 

 much as four-fifths of the protein has been removed, much 

 larger doses must be given before the phenomena of sensiti- 

 zation can be observed. 



The above substance, because of its specific ferment 

 action and its apparent chemical nature, I would designate 

 as anticancer globulin. 



