44 



THEORY OF COLLOIDAL BEHAVIOR 



of albumin served for all the experiments and was diluted before 

 the experiment to a 1 per cent solution. The concentration of 



pH 2.0 22 2.4 26 2.8 3.0 3.2 3.4 3.6 3.8 4.0 4.2 4.4 4.6 



FIG. 4. The ordinates represent the number of c.c. of 0.1 N HC1, H 2 SO 4 , 

 oxalic, and phosphoric acids required to bring 1 gm. of isoelectric crystalline 

 egg albumin to the pH indicated on the axis of abscissae. Enough H 2 O was 

 added to bring the albumin and acid to a volume of 100 c.o. For the same pH 

 the ordinates for HC1, HzSC^, and phosphoric acid are approximately as 1:1:3. 

 The ratio of HC1 to oxalic acid is a little less than 1 : 2 when pH is > 3.0. 



ammonium sulphate left in the solution was between M/ 1,000 and 

 M/2,000. The pH of the stock solution was about 5.20. By 



