46 



THEORY OF COLLOIDAL BEHAVIOR 



free is known from the pH of the albumin chloride solution, 

 namely, 1 c.c., since in the example selected the pH is 3.0 (Fig. 

 4). If 1 c.c. is deducted from 6 c.c. it is found that at pH 3.0 



19 

 18 

 17 

 16 

 15 

 14 

 13 

 12 



10 

 9 

 8 



pH 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2 3.4 3.6 3.8 40 4.2 44 4.6 4.8 



FIG. 5. Method of determining the amount of acid in combination with 1 gm. 

 of albumin from titration curve and pH curve. 



5 c.c. of 0.1 N HC1 are in combination with 1 gm. of originally 

 isoelectric crystalline egg albumin in 100 c.c. solution (Fig. 5). 

 A curve is constructed in which the abscissa are the pH while ^he 

 ordinates are the cubic centimeters of 0.1 N HC1 contained in 100 



