138 HUMAN INTESTINAL PROTOZOA IN THE NEAR EAST 



flies were enclosed under a glass with faeces of case Ure which 

 contained a fair number of cysts of E. histolytica and a leptothrix. 

 The flies were dissected twenty hours after the faeces had been 

 removed. The stomachs of all the flies contained faeces in which 

 cysts of E. histolytica occurred as well as the leptothrix which was 

 present in the stool. The loaded rectum of one fly was cut off and 

 examined separately. A few cysts of E. histolytica were seen. 

 Some of the cysts appeared quite normal and did not stain with 

 eosin, others appeared to be degenerate. In our previous publica- 

 tions we have shown that cysts which do not stain with eosin are 

 probably alive. 



(2) The same experiment was repeated with faeces from the 

 same case two days later with three flies. Dissected eighteen 

 hours after feeding, all three flies showed faeces in the gut, in which 

 the leptothrix was present but nothing definitely diagnosable as 

 cysts of E. histolytica were found. 



(3) Twelve flies were allowed to feed on faeces of case Turner 

 containing a fair number of cysts of E. histolytica. Dissected 

 eighteen hours after feeding the flies gave the following results : 

 (a) One fly had a fairly full gut and in the contents four undoubted 

 cysts were found, (b) Six flies had practically no faeces in the gut 

 and no cysts were discovered, (c) Five flies had absolutely empty 

 guts and no cysts were found. 



(4) Eight flies were fed on faeces of case McCaffrey which con- 

 ^tained a large number of cysts of E. coli and a small number of 



cysts of E. histolytica. The flies were dissected twenty-four hours 

 after feeding with the following results : (a) Four flies had empty 

 guts and no cysts wlte found, (b) Two flies had a little faeces in the 

 gut and eight-naici^ar cysts of E. coli were found, (c) One fly had 

 a good quantity of fasces in the gut, a fair number of cysts of 

 E. coli were present and one cyst of E. histolytica was seen. The 

 cyst did not stain with eosin. (d) One fly had a good quantity 

 of faeces in the gut and numerous cysts of E. coli which did not 

 stain with eosin. 



(5) Three flies were fed on stool of case McCaffrey and were 

 dissected forty-two hours after feeding, (a) One fly had a fair 

 amount of faeces in the gut and a corresponding number of E. coli 

 cysts which did not stain with eosin. (6) Two flies had empty 

 guts and no cysts were found. 



(6) Three flies were fed on stool of case McCaffrey and were 

 dissected seventy hours after feeding. The gut was empty in all 

 three flies and no cysts were discovered. 



