— i8 — 



(d) [ Goblet Cells Stained in Hsematoxylin and 



Eosin. 



The large intestine of a cat was macerated in S?>% alcoliol 24 to 

 48 lioiirs. Tease a small portion of the epithelium on a cover f^lass, 

 dry over the flame, and stain in htematoxylin and eosin. Blount 

 in balsam. 



Study under high power. Some of the cells observed 

 are ordinary columnar cells; others are distended with 

 mucinogen, the nucleus being pressed down into the lower 

 part of the cell ; these are the goblet cells. Sketch several 

 as seen under high power. 



(e) Isolated Ciliated Columnar Cells Stained in 



Haematoxylin and Eosin. 



The trachea of a cat was macerated in ?>3% alcohol. Tease a 

 portion of the epithelium on a cover glass, and stain in hicma- 

 toxylin and eosin. Mount in balsam. 



Study under high power. Observe that the majority 

 of the cells are columnar in shape, possessing an oval 

 nucleus. Notice particularl}'' the clusters of fine short 

 hairs adhering to the free end of some of the columnar 

 cells. A few goblet cells may be found. Sketch as seen 

 under high power. 



(f) Cross- Section of Stratified Ciliated Columnar 



Epithelium. 



The respiratory mucous membrane of a dog's nose was hardened 

 in mercuric chloride, stained in Delafield's hsematoxylin, embedded 

 in paraffin, and sectioned. Mount in balsam. 



Study under high power. This epithelium is com- 

 posed of three layers bf cells, the free cells being ciliated. 

 Sketch a portion of epithelial border. 



