-69 — 



fibrils will be seen. Fixed connective tissue cells are often 

 found on the network. Sketch under this power. 



(f ) Lymph Cells. 



Cover lilasses were di-awii over tlie freshly cut .surface of a lyuipli 

 gland, and fixe<l iu a .sohiKon of I] parts etlicr and 1 part absolute 

 alcohol for '.j liour. Stain iu lucuiatoxvlin for 1") minute.s, wasii in 

 water, counter-stain iu eosiu for r, minutes, ajrain wash iu water, 

 and dry hetweeu filter j)aper. Mount in Ijalsani. 



Study under high power. The greater proportion of 

 the cells seen liuve the shiipe and structure of the lympho- 

 cytes found in the blood. A few mononuclear and i)olynu- 

 clear cells will also be found. Sketch under high power. 



(g) Spleen. 



SuKdl pieci's fronrtiie sph'Pu of a dog were liardened in mer- 

 curic chiorid", stained in h.u-ax (•anuiiu\ emhedded in paratiin, and 

 sectioned. Fix to the slide, ami mount in l)alsam. 



Observe the capsule composed of connective tissue and 

 non-stri[)ed muscle cells. From the capsule trabeculse 

 pass into the gland, branching and forming an anastomos- 

 ing network ; their structure is similar to that of the can- 

 sule. Within the capsule two kinds of tissue are found, 

 the Malpighian corpuscles and the splenic }nilp. The; 

 former are composed of adenoid tissue, and are usuall}' 

 found surrounding the vessels. In the pulp a framework 

 of fine fibrils and cells is found, in the meshes of which 

 red antl wliite blood cells are seen. Sketch a portion under 

 high })uwer. 



(h) Cover Glass Preparation of Splenic Pulp. 



Cover Ldasses were drawn over tlie fresidy cut surface of a 

 spleen, and fixed in etiier-alcohol solmion f(jr 1 iiour. Stain iu 

 iuematoxylin and eosiu as (f ) of tliis lesson, and mount on l)alsam. 



Study under high power. Many red blood cells stained 

 with the eosin, and the various forms of white blood cells, 

 will be seen. Of the white blood cells the mononuclear 

 are found in much larger proportion than in circulating 

 blood. S^ietch the cells as seen under high power. 



