•I 1 1- 



rounded by a cortical ])ortion containing Graafian follicles 

 seen in ditFerent stages of development. The framework or 

 stroma is composed of filn'ousand non-striped muscle tissue. 

 A fully developed Graafian follicle consists of a dense sur- 

 rounding stroma; it is lined by the membrana granulosa 

 composed of several layers of small cells; in one j)ortion of 

 the follicle this lining is thickened to form the discus pro- 

 ligerus; in this discus the ovum is emliedded. I'hecavity 

 of the follicle is filled by the liipior folliculi. Other follicles 

 in diU'ereiit stages of development will also be observed ; in 

 some the ovum is suiToiinded l)y only a single layer of 

 cells; in others by several layers, while in still others the 

 cavity of the follicle may just be forming. The section 

 mav show a portion of a corpus luteum. If this is well 

 develo})ed, it will consist of a mass of quite large cells with 

 relatively small nuclei, between which a network of capilla- 

 ries will be seen. Sketch several Graafian follicles showing 

 diflferent stages of development, and a })_ortion of the corpus 

 luteum if present, as seen under high power. 



(d) Uterus. 



A liunian uterus was divided into small pieces, liardeued in 

 mercuric chloride, stained in Delaiield's luematoxylin, embedded in 

 l)araffin, and cross sections of the wall made. Fix to the slide, and 

 mouut in l)alsam. 



Studv first under low power. The uterus will show 

 mucous, muscular, and serous coats. The mucosa is com- 

 posed of loose fibrous tissue, and is covered b}' a layer of 

 ciliated columnar cells. In it are found the uterine glands, 

 simple or branched tubules lined b}' a layer of ciliated 

 columnar cells. The next coat is composed of non-striped 

 muscle tissue. Fibrous tissue is found between the muscle 

 bundles, also relatively large vessels. Sketch a portion of 

 the wall as seen under low power. • 



(e) Vagina. 



The vagina of a dog was hardened in absolute alcohol, stained 

 in borax carmine, embedded in jjaratlin, and sectioned. Fix to the 

 slide, and mount in balsam. 



Use the high power. The vagina has three coats, an 

 inner mucous, composed of stratified pavement epithelium 



