Methods for Maceratinsf Fresh Tissues. 



Ranvier's Alcohol. 



Place small [liccos of the tissue to be macerated in 33 

 % alcohol, in which they remain from 12 to 24 hours; then 

 transfer them to a 0.25 % osniic acid solution for two to 

 four liours. They can now l)e teased. 



This method is very useful for macerating- e})ithelial 

 tissue; for instance, the cells lining the intestinal canal or 

 the trache;i, for isolating the cells of the liver, etc. 



Caustic Potash Solution. 



Make a 30 % aqueous solution of KOH. Small 

 pieces are placed in this solution for 1.5 to 20 minutes. The 

 maceration is then interrupted by transferring the tissue 

 to a saturated aqueous solution of acetate of potash (it 

 takes about 60 parts of the acetate of potash to saturate 40 

 parts of water), to which a few drops of glacial acetic acid 

 have been added (the author adds five to six drops to 25 

 c. c. of the saturated solution). In about 80 minutes the 

 tissue is ready for teasing; it can, however, he kept along 

 time, several months, in the acetate of potash. 



This method is used for macerating non-striped and 

 heart muscle, also epithelial cells. 



Hydrochloric Acid. . . 



A -30 % aqueous solution is used; in it the tis- 

 sues remain for 12 to 24 hours, and are then washed in 

 flowing water for half an hour, when they are ready to be 

 teased. HCt is especially useful for isolating the tubules 

 of the kidnev- 



