126 



Nitric Acid (Gage). 



A 30 % aqueous solution is used; small pieces of the 

 tissue remain in the fluid about 24 hours, and are then 

 thoroughly washed in water. 



Strii)ed muscle fibres are well macerated l)y this 

 method. 



Sulphuric Acid (M. Schultz and Ranvier). 



Place the tissue to be macerated on the slide, add a 

 few drops of strong sulphuric acid, and cover with a 

 cover glass. In a few moments the cells can be separated 

 by gently tapping or pressing with a needle the top of the 

 cover glass. 



Useful for macerating hair, nail, and horny epidermis. 



Schultze's Mixture. 



One or two grams of crystals of chlorate of potash are 

 mixed with a little HNOg, only enough acid being used to 

 make a thick paste. In this the tissue to be macerated is 

 embedded for two or three hours, at the end of which time 

 the tissue may be removed to a slide and teased. If it 

 does not tease easily, embed again in the paste, repeating 

 at intervals of about thirty minutes, until it teases readily. 



This method brings out nicely the branched muscle 

 fibres. A frog's tongue may be used. 



Osmic Acid. 



A 1.0 % aqueous solution of osmic acid is very use- 

 ful for macerating retina, olfactory membrane, etc. The 

 tissues remain in the fluid from 24 to 48 hours, and may 

 then be teased. 



Macerated tissues are to be teased and mounted in 

 glycerine, glycerine gelatin, or gum glycerine. 



The glycerine gelatin may be pre^jared after Fol's for- 

 mula : 



Water, 42 c. c. 



Glycerine, 38 c. c. 



Gelatin, 7 grms. 



Carbolic acid, ..... 1 grm. 



Cut the gelatin in small })ieces, place the small pieces 

 into a clean beaker, and add the water. Warm over a 



