Methods for Decalcifying Tissues. 



Kahlden* gives the following general directions to be 

 observed when decalcifjang: — 



( 1 ) The tissues must first be well hardened in alcohol 

 or Miiller's fluid. 



(2) A large quantity of the decalcifying fluid needs to 

 1)6 used, and changed frequently. 



(3) After decalcification the tissues must be thoroughly 

 washed in flowing water for several days. 



(4) They are again hardened in graded alcohol, after 

 which they are ready for cutting. 



Nitric and Hydrochloric Acid. 



Use the following proportions: 



Nitric acid (10% aqueous solution) . 1 part. 



Hydrochloric acid (If's aqueous solution) 1 part. 



The decalcification is quite rapid, the fluid needs to be 

 changed every second or third day. The tissues are from 

 time to time taken from the fluid and tested b}' pushing a 

 needle into the bone, and if it enters easily and without 

 grating, the decalcification may ha considered complete. 

 They are then washed in flowing water, and hardened in 

 graded alcohol. 

 Ebner's Decalcifying Fluid. 



The following formula is taken from Behren's Ta- 

 bellen: 



Sodium chloride .... 2.5 grms. 



Water 100.0 c.c. 



Alcohol 500.0 c.c. 



Hydrochloric acid .... 2.5 c.c. 



*Teclinik der Histologischeii Uutersiicliuug patolo^isch-ana- 

 tomiscber Pra-parate. Third edition, page 13. 



