—133— 



This solution decalcifies very slowly, but without in- 

 jury to the tissues. Large quantities (10 to 20 times the 

 hulk of the tissue) need to be used, and one or two c.c. of 

 liy(h-()chIoric acid are daily added to the lluid until decal- 

 cification is complete. Wash very thoroughly in flowing 

 water, and harden in graded alcohol. 



Haug's Chrom-Osmium Solution. 



Osmic acid (1% aqueous solution) . 10 c.c. 

 Chromic acid (1% aqueous solution) . 25 c.c. 

 Water 60 c.c. 



Useful lor decalcifying the cochlea; it acts very slowly. 

 Wash in flowing water, and harden in alcohol. 



*Lepkowski's Method. 



Hniall {)ieces of tooth or bone are })laced in the follow- 

 ing solution : 



Chloride of gold (1% aqueous solution) H parts. 

 Formic acid ..... 8 parts. 



The tissues are decalcified in two or three days. The 



chloride of gold is deposited in the dentinal tubules or 



canaliculi, so that the tissue will be both stained and 



decalcified at the same time. 



*Anatomischer Anzeiger, 1892. 



