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Flemming's Safranin Solution. 



Safranin ..... 1 grm. 



Absolute al(H)liol ... 10 c. c. 



Aniline water • . . . . 90 c. c. 



The aniline water is prepared by shaking 5 to 8 c. c. 

 of aniline oil with 100 c. c. of distilled water, and filtering 

 through a icet filter paper. Dissolve the safranin in aniline 

 water, and add the alcohol. Filter before using. 



The steps for staining are as follows : — 



Harden tissues in Fleinmins's or Hermann's sohition. 



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Enil)ed in ])araffin. 

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Cut very thin sections, and fix to cover glasses. 



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Remove paraffin with tohiol or xylol, and, after rinsing 

 sections in alcohol, transfer into distilled water. 



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Stain in Flemming's safranin solution for 24 to 48 hours. 



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Rinse in distilled water. 



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Wash tlioronghly in 95% alcohol (200 c. c.) to 

 which 1 or 2 drops of HCl have been added. 



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Wash in alcohol, clear in oil of bergamot, pass through 

 xylol, and mount in balsam. 



In tissues hardened and stained in the above way, the 

 chromatic network of the nucleus is well stained; this 

 metliod is largely used for staining cells in process of 

 division. 



Gentian Violet Solution (Ehrlich and Bizzozero), Taken 

 from Lee's Vade Mecum. 



Gentian violet .... 1 part. 



Alcohol ..... 15 parts. 



Aniline oil ..... 3 parts. 



Distilled water .... 80 parts. 



