Boehmer's 

 Haematoxylin. 



— 163— 



Ehrlich's 

 Haematoxylin. 



Delafield's 

 Haematoxylin. 



Tissues nijiy be liuidcncil in alcoliol, Mailer's 

 fluid, or bicliluride of nieiriirv. 



EiiiIxmI ill 



]''iiil)eil ill 



rar.idiu. Celini.liu. Parafiiii. Celloidiii. 



Fix sections to 

 cover glass. 



V 

 Remove 



paralfia. 



V 



Fix sectioTis to 



i-ovcr glass. 



I 

 V 



ReiiiKve 



liaiMlliii. 



Distilleil water. Distilled water. 



Stain en manse for L'4 



to 48 hours. 



I 

 V 



Wash ill acid alcohol 



2 — hours. 



Ill flowing tap water 

 1 — 2 hours. 



Dehydrate. 

 Embed in 



V V Paraffin. Celloidin. 



Stain from 10 to 30 Stain for ]6 to GO I 



minutes. minutes. t-- ^ ,• 



rix sections to 



I I cover glass. 



^ ''I 



A\ ash in 0.ri% potash Wasli in tap water V 



alum 5 minutes. 10 to lo minutes. Remove 



, . paralliii. 



V V |, \ 

 Distilled water. Distilled water. Distilled water 



V 



Stain in eosiu for ,5 to 10 minutes, or in acid fuchsin 

 for 10 to 15 minutes. 



"Wash in distilled water. 



Wash and dehydrate in alcohol. 



Clear in oil of bergamot. 

 I • I 



V V 



Paraffin sections fixed to 

 cover glass. 



Celloidin sections. 



Pass through xylol, and mount 

 in balsam. 



Mount in balsam. 



