— 164 — 

 Orange G and Haematoxylin (Kawitz). 



The tissues should be lianloneil in ifnsolute alcohol, mercuric 



chloride, or picric acid. 



i 



Embed in paratiin, and lix sections to cover glass. 



I 

 \' 



Kemove paraliin, and brinj,' sections into distilled water. 



I 

 V 



Stain in a saturated aqueous solution of Orange G (filtered; 



for about 24 hours. 



I 



Rinse in distilled water. 



i 



Stain in Boehmer's ha'matoxylin solution for about 5 



minutes. 



I 

 ^■ 



AVash in distilled water. 



I 



V 



Dehydrate in alcohol. 



^' 



Clear in oil of berganiot. 



I 

 y 



Pass througli xylol, and mount in balsam. 



Tliis is certainly one of the best of double stains, 

 especiall}^ for glandular tissues. The nuclei are stained 

 with the hpematoxyliu, the protoplasm with orange G. 



Safranin and Lichtgruen (Benda). 



Tissues hardened in Flemming's or Hermann's solution 

 are embedded in paraffin, and sections fixed to the cover 

 glass. Eemove paraffin, and bring sections into distilled 

 water. 



Stain for about 24 hours in the following safranin 

 solution : — 



Safranin ..... 1 grm. 



Aniline water . . . . 90 c. c. 



Absolute alcohol . . 10 c. c. 



