— 166— 



4. The sections are now transferred to distilled water, 

 in which the sections again assume a l)lue color, of a mucli 

 lighter hue, however. In the distilled water the sections 

 remain about 10 minutes. 



5. Connter-stain in a saturated aqtieous solution of 

 safranin for about 30 minutes. 



6. Rinse in distilled water. 



7. Wash, and dehydrate quickly in 95''u and absolute 

 alcohol. 



8. Clear in oil of l)ergamot, pass sections through 

 xylol, and mount in balsam. 



The axis cylinders are all stained blue, tlu^ myelin a 

 yellowish-red or orange color, and ;dl nuclei red. 



Carmine and Aniline Blue ( Duval). 



Tissues are stained in borax caiinine in the usual way, 

 are then embedded in paraffin, sectioned, and sections fixed 

 to cover glasses. Remove paraffin from sections, and Ijring 

 them into absolute alcohol. 



Stain for 10 to 20 minutes in — 



Aniline blue, saturatetl alcohol solution 10 drops. 



Absolute alcohol .... 10 c. c. 



-« Wash, and clear sections in turpentine, jjass through 

 xylol, and mount in balsam. 



Tlie central nervous system is well stained after this 

 method; the tissues may be hardened in l)iclili)ride of 

 mercury. The connective tissue elements are stained blue, 

 the nerve cells and axis cylinders ;i reddish-violet. 



Borax Carmine and Indigo Carmine fNorris and Shakes- 

 peare, taken from Bolim and Op[>ers TusliciihiirJi 

 tier mlkroskopisrJicn TerJinic). 



Sol. A. Grind in a mortar. 



Carmine 2 grms. 



Borax 8 grms. 



Distilled water . . . . 180 c. c. 



Allow to stand for 24 hours, then filtor. 



Sol. B. Rub up in mortar. 



Indigo carmine . . . . cS grms. 



Borax 8 grms. 



Distilled water . . . . 1,30 c. c. 



Allow to stand for 24 liours, then filter. 



