cover glasses and crushed, after wliich procedure the 

 covers are quickly drawn apart; or the cover glasses may 

 he drawn over the freshly cut surface of the tissue. Ihese 

 preparations may after fixing be stained after any one of 

 the following metliods: — 



Haematoxylin and Eosin Stain. 



Tlic prei)arati()ns are fixed for about 1 hour in ether 

 and alcohol or are exposed to a temperature of 100^ to 110° 

 C. for about 45 to 60 minutes, stained in Bochmer's luema- 

 toxylin for 20 to 80 minutes, washed in flowing water, 

 counter-stained in cosin for 5 minutes, again washed, and 

 quicUii dried between filter paper. Mount in balsam. 



All nucK'i are stained blue, i>n)to])la>m and eo.-ino- 

 phile granuhs red. 'i'he other granules are not stained. 



Ehrlich's Neutrophile Mixture — 



Orange G (salui'ated :i(|ueous solution), . 130-135 c. c. 

 Acid t'liclisin (saturated aciueous solution), 80-165 c. c. 

 Methyl green (saturated acjueous solution), 125 c. c. 



Distilled water 300 c. c. 



Alcohol (absolute), 200 c. c. 



Glycerine, 100 e. c. 



Mix orange G, acid ("uehsiii. water, and absolute alcohol 

 in a bottle, add slowly ami while shaking the methyl 

 green. The glycerine is then added. For staining in 

 .Ehrlich's neutrophile mixture, the pre})arations need to be 

 hardened in ether and alcohol for about one hour, or fixed 

 at a temperature of 100' to 110' C. for 15 to 30 minutes. 

 Float the preparation on a small quanitity of the stain for 

 about 15 minutes, wash in fiowing water, dry })etween 

 several filter ])ai)ers. and mount in balsam. The red cor- 

 puscles should have a reddish-brown color (brick color), 

 all nuclei green, the eosinophile granules red, and the 

 neutrophile gi'anules violet-red. 



Chenzinski's Stain. 



Methylene blue (saturated aqueous sol-^tion), . 40 parts. 



Eosin (1% sol. in 70;'^ alcohol), . . . 20 parts. 



Distilled water, 30 parts. 



Glycerine, 10 parts. 



