METHODS OF EXAMINATION 543 



staining with safranin and light-green is also a very suitable one. 

 Sections are stained for several hours in a saturated solution 

 of safranin in aniline oil water (p. 98), they are then washed 

 in water and decolorised in a \ per cent solution of light-green 

 in alcohol till most of the safranin is discharged, the nuclei, 

 however, remaining deeply stained. In this method the nuclei 

 of the amoebae are coloured red (like these of the tissue cells), 

 the protoplasm being of a purplish tint. 



