564 KALA-AZAR 



often going on for several years, and is extremely fatal, in 

 fact, it is difficult to say if recovery can ever take place. 

 Post mortem, there is little to note beyond the enlargement of 

 the liver and spleen, but in the intestine, especially in the 

 colon, there are often large or small ulcers, and there is evidence 

 of proliferation in the bone marrow, the red marrow encroaching 

 on the yellow. 



In a film made from the spleen and stained by Leishman's 



Fia. 170. Leishman- Donovan bodies from spleen smear. x 1000. 



stain, the characteristic bodies can be readily demonstrated 

 (Fig. 170). They are round, oval, or, as Christophers has 

 pointed out, cockle-shell shaped, and usually 2*5 to 3 -5 //, in 

 diameter, though smaller forms occur. The protoplasm stains 

 pink, or sometimes slightly bluish, and contains two bodies 

 taking on the bright red colour of nuclear matter when stained 

 by the Romanowsky combination. The larger stains less 

 intensely than the smaller, is round, oval, heart-shaped, or 

 bilobed, and lies rather towards the periphery of the body in 

 the region of the " hinge " in the cockle-shaped individuals. 



