ADIPOSE TISSUE. 87 



lar extensions, as seen in section ; the smaller fasci- 

 culi, which are separated by areolar tissue, corre- 

 sponding with the whole of one of the caudal ten- 

 dons of the mouse or rat. 



Preparation 15. Cell-spaces of Tendon. To 

 show the cell-spaces in which the above-mentioned 

 tendon-cells are contained: 



As before, break off the end and draw out some of 

 the tendons of a mouse's tail (that previously used 

 will still yield a sufficient number). Then hold the 

 tendons in a shallow dish of distilled water, and 

 with a medium-sized camel-hair pencil brush them 

 firmly from end to end. six or eight times. Remove 

 them now from the water, and immerse them in a 

 large watch-glass of nitrate of silver solution (J per 

 cent.) for fifteen minutes; then place in a glass ves- 

 sel of water and expose to sunlight. As soon as they 

 are well browned, pieces may be cut off, laid straight 

 in water upon a slide, covered, and the water then 

 drawn off and replaced by glycerine; after which 

 the cover-glass may be fixed in the usual way. 



Preparation 16. Epithelioid covering of Ten- 

 dons. The object of first brushing the tendons is 

 to remove the layer of flattened cells which covers 

 the surface of each, and which, if allowed to remain, 

 prevents the silver solution from properly acting 

 upon the deeper parts of the tissue. To show this 

 layer, another set of tendons may be treated with 

 the silver solution in a similar way, but with a 

 minute's immersion and without previously brush- 

 ing them, when it will probably be found that the 

 superficial epithelioid stratum is alone apparent. 



Preparation 17. Adipose Tissue. The sim- 

 plest way of showing the fat vesicles is by teasing 

 out a small portion of the tissue in a drop of salt 

 solution; taking the precaution of putting a narrow 

 slip of blotting-paper on either side to avert the 

 pressure of the cover-glass. 



But a far better way of demonstrating the struc- 

 ture of the tissue generally, consists in the employ- 



