THE GASTRIC GLANDS. 213 



mixture ; spirit alone also answers very well. The 

 duodenal end is tied up and a glass canula is fast- 

 ened into the oesophageal end. This is connected 

 by an India-rubber tube with a glass tube which 

 passes to the bottom of a bottle containing the "hard- 

 ening fluid. A second tube passes just through the 

 cork of the bottle, and by blowing through it the 

 fluid is forced into the stomach. When the organ 

 is moderately distended the India-rubber tube is 

 clipped, to prevent any of the liquid being forced 

 back into the bottle by the contraction of the mus- 

 cular walls of the stomach ; the gullet is then secured 

 by a ligature, and the whole organ is immersed in a 

 large bottle or covered beaker filled with the same 

 mixture of chromic acid and spirit. After twenty- 

 four hours it should be opened and put into fresh 

 fluid; or, if it is not desired to keep the whole of 

 the organ, small pieces only from different parts are 

 so transferred. (Indeed, if the stomach be too large 

 to harden as a whole, or difficult to be cleared of its 

 contents, small pieces may be cut out from the fresh 

 organ, pinned out on a piece of cork or cake of wax, 

 and thus immersed in the spirit and chromic mix- 

 ture). In two or three days more the tissue is hard 

 enough, if the spirit be of the strongest, to cut sec- 

 tions from, and small pieces may accordingly be 

 embedded with this view. The sections, "which 

 should comprise all the coats of the organ, are to be 

 stained with logwood and mounted in dammar as 

 usual. They will show well enough the relative 

 thickness of the several layers and many of the 

 structural points, but for making out distinctly the 

 structure of the mucous membrane and the characters 

 of the cells which occupy the gastric glands it is 

 necessary to make thinner sections than are easily 

 obtainable in conjunction with the muscular coat. 



Preparation 4. Gastric glands. With this 

 object, then, small pieces of the fresh mucous mem- 

 brane are taken from two distinct parts, one from 

 near the pylorus and the other from the cardiac 



