214 PRACTICAL HISTOLOGY. 



fundus, and placed at once in absolute alcohol. 

 When hardened they are embedded separately, and 

 vertical sections, as thin as possible, are made. 

 Those from the pyloric piece are to be stained with 

 logwood and mounted as before in dammar, but one 

 or two of the thinnest may be selected and mounted 

 in glycerine. Those from the cardiac piece are to be 

 stained and prepared in three different ways. In 

 the first place, two or three may be treated as just 

 recommended for those from the pyloric piece. 

 Secondly, other two or three are to be placed for 

 twenty-four hours in a weak solution of carmine 

 (the carmine solution, p. 207, diluted five times). 

 Thirdly, one or two others are to be stained with 

 aniline blue. There are different kinds of this sold 

 in the shops ; that known as Nicholson's No. 1 gives 

 good results. A one per cent, solution is used, and 

 the sections are left in for about thirty minutes. 

 They are then placed in a watchglass containing a 

 mixture of glycerine and water, equal parts, and are 

 finally mounted in glycerine. The carmine-stained 

 sections are also mounted in glycerine, after having 

 been rinsed in water. 



These different modes of staining bring out dis- 

 tinctly the differences between the various kinds of 

 cells found in the peptic glands. In the logwood 

 preparations the peptic cells will be found stained 

 rather less than the rest, whereas in the sections 

 stained by carmine and aniline they are colored 

 more deeply. This is especially the case in the ani- 

 line preparations, where the peptic cells are stained 

 of a deep blue, whilst the other cells remain almost 

 colorless. 



Preparation 5. Horizontal sections. Besides 

 the vertical sections of the mucous membrane others 

 are to be made parallel to the inner surface, and 

 therefore so as to cut the glands across. To effect 

 this a small piece of each region is embedded, with 

 the part which corresponds to the inner surface of 

 the stomach placed opposite the end of the mould. 



