BLOODVESSELS OF THE STOMACH. 215 



It is then gradually cut into slices, which will, of 

 course, comprehend in succession first the mouths of 

 the glands, then their necks, and finally the deeper 

 parts. The sections so obtained are to be stained 

 and mounted in the same way as the vertical sec- 

 tions. 



Preparation 6. Cells of the glands, iso- 

 lated. In addition to studying them in sections 

 in this way the cells of the glands are also to be 

 studied in teased-out preparations of the fresh mu- 

 cous membrane. If prepared in serum they will 

 show T better than by any other method the characters 

 of the different kinds of cells. The cells will be 

 more readily obtained separate if a small piece of 

 the membrane is placed in J per cent, bichromate of 

 potash solution for twenty -four or forty-eight hours, 

 but the cells are apt to be somewhat altered, and the 

 columnar cells of the general surface and mouths of 

 the glands to become transformed, by the swelling 

 and escape of their contained mucus, into goblet- 

 cells. 



Preparation 7. Bloodvessels of the stomach. 

 But vertical and horizontal sections of an injected 

 stomach are to be made. This may be obtained 

 from the animal which was injected entire: If it 

 were a rat, the preparations are to be made from the 

 pyloric half of the organ, since in this animal the 

 cardiac part has a non-glandular mucous membrane 

 with stratified epithelium like that of the gullet. 

 The vertical sections need not be very thin ; they 

 are improved by being placed for a few minutes in 

 dilute logwood, so as to become slightly colored, 

 before being mounted in dammar by the ordinary 

 process. Instead of cutting horizontal sections a 

 small piece of the injected stomach may, if from a 

 small animal, be simply mounted flat with the inner 

 surface uppermost, without staining. 



Preparation 8. An attempt may be made to inject 

 the lymphatics of the gastric mucous membrane with 



