NERVES OF INTESTINE. 219 



process. The sections are placed, after the cacao-butter 

 has been extracted from them by warm oil of cloves, first 

 in spirit, and then in water, and are finally mounted in 

 glycerine. 



In the two teased preparations serum and osmic 

 many of the columnar epithelium cells will be founi to 

 contain fatty globules of various sizes (stained blaok in 

 the osmic preparation). Similar, but for the most part 

 smaller particles will also be found in the numerous 

 lymphoid corpuscles which are set free from the retiform 

 tissue of the mucous membrane by the process of teasing. 

 In the sections the epithelium cells and the lymph cor- 

 puscles will be observed, in situ, in the same condition, 

 viz., containing blackened fatty particles, and moreover 

 the cleft-like central lacteal in the middle of each villus 

 will be found to contain similar globules. Hence we infer 

 that the tatty matters are first taken up from the cavity 

 of the intestine by the columnar epithelium cells ; that 

 they are transmitted in some way from these to the 

 amoeboid lymph cells, and that these again convey them 

 to and discharge them into the central lacteal. 



Preparation 3. Vessels of the small intes- 

 tine. The bloodvessels of the small intestine are to 

 be studied by aid of vertical sections of the injected 

 gut. The sections may be lightly stained with 

 logwood. 



The lymphatics (lacteals) may perhaps be seen in thin 

 sections of the uninjected preparations as cleft-like spaces 

 in the villi and in the substance of the mucous membrane, 

 and surrounding the bases of the lymphoid nodules which 

 make up the Peyerian patches. It is not an easy matter 

 to inject those of the mucous membrane, although the 

 larger plexuses of the submucous and muscular coat can 

 be more easily demonstrated. 



Preparation 4. Nerves of the intestinal 

 wall. The nerves of the intestinal canal form a 

 very interesting subject of study, comprising two of 

 the closest and most richly gangliated plexuses of 

 pale fibres which are met with in the animal body. 

 They may, moreover, by following the method here 

 to be described, be shown without any great diffi- 



