LYMPHATICS OF THE LIVER. 227 



of the biliary ducts. A rabbit is killed by bleeding, 

 the abdomen opened, and the common bile-duct 

 sought for close to the portal vein ; a ligature is 

 passed round it and a small piece of card being 

 placed under as a support, and to separate it from 

 the accompanying vessels, a snip is made into it, 

 and a glass canula is inserted, and having been 

 passed along the duct as near to the liver as possible 

 is tied in. The cystic duct is ligatured to prevent 

 the injection from passing into the gall-bladder. In 

 the next place the canula is filled with warm Berlin 

 blue solution by means of a fine pipette ; the (pre- 

 viously filled) supply tube is attached, the clip on 

 this opened, and the pressure gradually raised to 

 about two inches of mercury. The blue fluid, driving 

 whatever bile there happens to be left in the ducts 

 before it into the lobules, penetrates first into the 

 interlobular bile-ducts, and from these into the outer 

 parts of the lobules, forcing the bile more and more 

 towards the centre ; here of course there is no escape 

 for it, except that a little may pass into the lym- 

 phatics and bloodvessels through their walls. Hence 

 it will be understood that the injection can only be 

 made to fill the intercellular biliary passages in the 

 outer part of each lobule. The injection should be ' 

 persevered with for about half an hour ; the bile- 

 duct may then be tied and the injecting apparatus 

 removed ; after which the liver is cut out entire, 

 without injuring it in any way, and placed in strong 

 spirit. In twenty-four hours it is cut in pieces, and 

 the spirit changed, and in less than a week the 

 pieces will be hard enough to cut. The sections 

 may be stained slightly with logwood. 



Preparation 4. Lymphatics of the liver. 



The lymphatics of the liver are injected through a fine 

 canula stuck obliquety into the superficial part of the 

 organ immediately beneath the capsule. Either solution 

 of Berlin blue or alkanet may be used. The part should 

 be quite fresh. If the injection be persisted in for a long 

 while, the fluid may flow out both by the lymphatics 



