138 



avoided throughout the whole course of manipulation. If 

 the sections dry they are most likely ruined. 



After mounting, put the slides into the paraffin oven for 

 several hours to harden the balsam. The label should bear 

 not only the name of specimen but, advisably, the name of 

 stain used and also the name of fixing agent. The date of 

 mounting should be added, so that the sections may serve as 

 tests of the value of the method of fixing and staining. 

 Some stains do not hold well when used after certain fixing 

 agents. 



RECAPITULATION. 



As a convenient guide for the beginning student the 

 course of procedure described in the preceding pages is here 

 recapitulated in its proper sequence. 



FOR MATERIAL TO BE STAINED IN SECTIONS ON SLIDE. 



1. Kill and fix fresh material with fixing agent (p. 124). 



2. Wash out fixing agent with 70^ alcohol (pp. 125, 126), 



3. Remove to 85^ alcohol for keeping, or for beginning 

 hardening (pp. 125, 126). 



4. Harden in 95^ alcohol and absolute alcohol (p. 127). 



5. Clear in xylol, cedar-wood oil or other clearer (p- 

 127). 



6. Infiltrate with paraffin in paraffin oven (p. 127). 



7. Imbed (p. 128). 



8. Cut sections (p. 129). 



9. Fasten sections on slides with Mayer's albumen and 

 water (p. 132). 



10. Remove paraffin from sections with xylol or other 

 agent (p. 132). 



11. Remove xylol with absolute alcohol (p. 133). 



12. Carry through 90^ alcohol and 70^ alcohol (p. 133). 



