28 



that is, 0*3 c.c. of the oyster, showed, as near as could be 

 counted, 6400 colonies, that is, about 40,000 B. typhosus for 

 the whole fish. As regards the oysters kept in sea water in 

 the tub, the tub and the sterile sea water were changed after 

 one, two, three, five, six, and seven days ; oysters of the wet 

 lot were analysed one day after change, two, five, six, seven, 

 and nine days ; of the dry lot we analysed after one day dry, 

 two, three, five, six, seven, and nine days dry. 



One day after change, oyster 3 (wet) total amount of 

 fluid 3 c.c., ^Q c.c. for one Drigalski plate, contains 46 colonies 

 of B. typhosus this would amount to 46 x 30 = 1380 B. 

 typhosus per whole oyster. 



One day dry, oyster 2 total amount of fluid 4 c.c., 

 ^ - c.c. for one Drigalski plate, contains 1000 colonies of 

 B. typhosus this would amount to 1000 x 40 = 40,000 B. 

 typhosus per whole oyster. 



Two days after change in sterile sea water, oyster 5 (wet) 

 total fluid 2 c.c. ; two days, oyster 4 (dry) total fluid 

 1 c.c. ; of each oyster -fa c.c. for one Drigalski plate. 



Total number of colonies of B. typhosus in Drigalski 

 plate of oyster 5 were 22, that is, 22 x 20 = 440 B. typhosus 

 per whole oyster ; Drigalski plate of oyster 4 contained 377 

 colonies of B. typhosus this amounts to 3770 B. typhosus 

 for the whole oyster. 



Three days dry, oyster 6 total fluid 1 c.c. ; -fa c.c. was 

 used for one Drigalski plate, which developed 70 colonies of 

 B. typhosus this amounts to 700 B. typhosus for the whole 

 oyster. 



Five days after change in sterile sea water, oyster 7 

 total fluid a little under 2 8 c.c., of this made two Drigalski 

 plates each with -fa c.c. 



Total number of colonies of B. typhosus in the two plates 

 was six (two in one, four in the second plate) this would 

 amount to about 82 B. typhosus for the whole oyster. 



Five days dry, oyster 8 total amount 1 5 c.c. ; -fa c.c. 

 produced in a Drigalski plate 10 colonies of B. typhosus 

 this would amount to 150 B. typhosus per whole oyster. 



Six days after change in sterile sea water, oyster 9 total 



