58 



say, 44 millions per 1 gram of faeces a considerably smaller 

 number than in Experiment VII ; in fact, barely the tenth 

 part of the number used in the latter experiment. 



Into this infected sea water were placed the remaining 

 ten oysters, and they remained herein for 48 hours. After 

 this period the infected sea water as also one oyster (3) were 

 analysed, the remainder were well rinsed under the tap and 

 then transferred to a fresh tub and fresh sterile water, and 

 this procedure was repeated after a further day, also after 

 two, four, six and seven days ; at each of these periods of 

 change, oyster or oysters were taken out and analysed. 



The sea water, 48 hours after infection, contained 4110 

 B. coli communis per 1 c.c. The sea water in tub one day 

 after change contained 40 B. coli communis per 1 c.c. 

 that is, the originally sterile sea water in the tub to which 

 the infected oysters had been transferred. The sea water 

 twice changed contained B. coli per ~- ff c.c. 



Oyster 3 having been kept 48 hours in the fsecally infected sea 



water, contained in its body 650 B. coli communis. 

 ,, 4 having been kept 1 day in sterile sea water, contained 



84 B. coli communis. 

 5 having been kept 2 days in sterile sea water, contained 



600 B. coli communis. 

 6 having been kept 3 days in sterile sea water, contained 



484 B. coli communis. 

 7 having been kept 5 days in sterile sea water, contained 



52 B. coli communis. 

 8 having been kept 7 days in sterile sea water, contained 



48 B. coli communis. 

 9 having been kept 7 days in sterile sea water, contained 



B. coli communis per | oyster. 

 10 having been kept 8 days in sterile sea water, contained 



18 B. coli communis. 

 11 having been kept 8 days in sterile sea water, contained 



B. coli communis per -J- oyster. 



One of the oysters, not analysed, died two days after the 

 commencement of the experiment. 



