PKACTICAL HISTOLOGY [ill 



NOTES. 



The following solutions are used for staining films which 

 have been fixed either by heat (cp. 4) or by fixing fluids (cp. 

 below). 



1. Hcematoxylin and eosin. Make Ehrlich's acid hserna- 

 toxylin (cp. p. 307) dissolving *5 gram eosin in the water. Films 

 stain in this in half-an-hour to two hours. 



2. Chenzinsky's eosin and methylene blue. 



Saturated aqueous sol. methylene blue 40 c.c. 



0'5 p.c. eosin sol. in 70 p.c. alcohol 20 



Distilled water 40 



This stains slowly, requires several hours to a day ; the fluid 

 should be filtered before use. 



3. Ehrlich's ' triacid ' fluid. Add in order, shaking between 

 each addition. 



Saturated aqueous solution of Orange-G. 14 c.c. 

 Acid Magenta 7 



Distilled water 15 



Absolute alcohol 25 



Saturated aqueous solution of methyl-green 12 -5 



Glycerine 10 



This stains in about five minutes. It is chiefly used to 

 distinguish 'neutrophil' granules staining violet, from oxyphil 

 granules staining a brownish red. 



Fixing wet films. In dry films, the white corpuscles and 

 platelets are flattened; to avoid this the films are placed a 

 second or so after they have been made with the film surface 

 downwards, in a fixing agent. 



The following fixing agent may be used : formol, 10 parts, 

 95 p.c. or absolute alcohol, 90 parts. In a little of this fluid the 

 cover-glass is placed, film surface downwards, for 5 to 10 minutes \ 



