LESSON Y. 



STAINING AND MOUNTING UN-IMBEDDED 

 SECTIONS. 



1. Staining sections with hsematoxylin and 

 with picro-carmine. Sections are given you in 

 water 1 . Place a drop or two of hsematoxylin 2 

 (Ehrlich's or Delafield's) in a watch-glass; dilute it 

 with distilled water just so far that a section placed 

 in it can be easily seen. Fill another watch-glass 

 about Jrd full with picro-carmine. 



Place separately on a slide a drop of each of the 

 fluids, and examine them with a low power of the 

 microscope. If particles are visible in either, filter it. 

 A small filter may be taken, folded, held in forceps over 

 a clean watch-glass ; sufficient staining fluid will filter 

 in about a minute. 



Lift up a section on a glass rod 1 to 2 mm. in 

 diameter 8 and place in the hsematoxylin. Transfer 



1 Sections of spleen hardened in potassium bichromate may be 

 taken for 1 to 8, and the results compared. 



a For the method of preparing the staining fluids see p. 307. 



3 Instead of a glass rod, a glass tube finely drawn out may be 

 used, the end being closed by fusing in a Bunsen flame. 



32 



