40 PRACTICAL HISTOLOGY [v 



6. When it is desired to keep sections mounted in glycerine, 

 they may be treated in the following way. Place the slide and a 

 small bottle of glycerine-jelly on a warm bath. There should be 

 no glycerine beyond the edges of the cover-slip, but if there is, 

 remove it with blotting-paper. With a small brush, brush a 

 little glycerine-jelly round the edges of the cover-slip. Put the 

 slide aside for a day or more, then brush gold size (zinc white, 

 Brunswick black, Canada balsam will also serve) over the 

 glycerine -jelly. 



7. Mounting in glycerine-jelly. Place at about 

 38 C., (a) a small bottle containing glycerine-jelly, 

 (b) the sections in water, (c) a lifter, (d) a clean slide 

 and cover-slip. When the jelly is melted, place a 

 section on the slide, run off the water, place a drop 

 of glycerine-jelly on the section ; leave in the warm for 

 a minute, gently moving the section with a warm glass 

 rod or lifter. Cover, and leave for another minute in 

 the warm, gently press the cover- slip, or the layer of 

 jelly may be too thick to allow examination under the 

 high power. The essential point is to keep everything 

 warm; if this is borne in mind, the details may be 

 varied in obvious ways. 



When the jelly has set, remove that at the edges of 

 the cover-slip and surround with gold size or other 

 cement. 



8. Double staining with haematoxylin and 

 eosin. Arrange watch-glasses as in 1, omitting that 

 containing picro-carmine and adding one containing 

 a saturated solution of eosin in 75 p.c. alcohol. Stain 

 rather deeply with hsematoxylin ; after the stained 

 section has been placed in 50 p.c. alcohol, place it 

 in the eosin solution for one minute, then clear 

 in 75 p.c. alcohol. Pass through 95 p.c. alcohol with 



