44 PRACTICAL HISTOLOGY [VI 



since turpentine is apt to dissolve some of the stained fatty 

 substances. 



2. Staining free in fluid, sections imbedded in paraffin. Take 

 two or three sections and dissolve the paraffin with turpentine 

 as in 1. Take up the sections on a thin glass rod, touch 

 blotting-paper with them to remove excess of turpentine and 

 place in 95 p.c. (or absolute) alcohol in a watch-glass. Leave for 

 5 to 10 minutes, then pass through successively weaker alcohols, 

 stain and mount as in Less. v. 1. 



NOTE. Sections may be fixed in 3, 4, 5 to the 

 cover-slip instead of to the slide, but the beginner will 

 find the slide easier to handle. He will also find 

 turpentine easier to use than xylol since it evaporates 

 less quickly and there is thus less chance of the section 

 becoming dry; turpentine is not so good as xylol for 

 the cover-slip method since it is apt to leave a slight 

 film on evaporation. 



3. Mounting serial stained sections. Place a 

 small drop of collodion dissolved in clove oil, on a slide. 

 With a small brush (or with the finger) rub the drop so 

 that it forms a very thin film over a portion of the slide a 

 little larger than a cover-slip. Take the ribbon of nine 

 sections 1 given you, and cut it with a scalpel into three 

 series of three sections each. Place these on the film 

 of collodion so that the second series of three is below 

 the first, and the third below the second. Press a small 

 brush lightly on the centre of each section and roll it 

 to right and left, so as to flatten the sections and make 



1 A piece of small intestine hardened in mercuric chloride may 

 be taken ; stained with hsematoxylin for 3, 4; unstained for 5. 



