Vl] STAINING IMBEDDED SECTIONS 45 



them stick to the glass. Place the collodion side 

 uppermost, on a warm bath at a temperature just 

 sufficient to melt the paraffin. When the paraffin is 

 melted, dip the slide into a bottle of turpentine, for 

 one to two minutes. Then wipe the turpentine from 

 the lower surface of the slide, and stand the slide up to 

 drain. Wipe away the turpentine on either side of the 

 sections. Place a line of dilute balsam on a cover-slip 

 near one edge ; let this edge first touch the slide, and 

 gently lower the cover-slip so that the balsam flows over 

 the sections. 



4. Flattening folded sections, a. Clean the 

 slide by pouring on it a drop or two of strong spirit, and 

 rubbing with a clean cloth. Place a flat basin of distilled 

 water over a small flame, regulating the flame so that 

 the temperature of the water is 35 to 37 C. Place the 

 sections on the water, they will spread out and become 

 quite flat. Dip the slide in the water obliquely, 

 draw the sections to it with a needle, hold the upper 

 edge of the paraffin on the slide with the needle and 

 gently lift the slide out of water, with the sections on 

 it. Stand it up to let the water run off, so that the 

 sections are in contact with the glass, place between 

 smooth pads of blotting-paper, and touch lightly to 

 remove most of the remaining water and to make the 

 tissue adhere to the glass. Place on a bath at about 

 35 C. for half-an-hour or longer to dry without melting 

 the paraffin. Then place on a warmer bath to melt 

 the paraffin and mount in balsam as in 3. 



b. Clean a slide ; pour water on it, place the sections on the 

 water. Place the slide on a warm bath. As the water becomes 



