72 PRACTICAL HISTOLOGY [X 



seen. In about a quarter of an hour, wash in 95 p.c. alcohol, 

 tease out one end on a slide with fine needles, transfer to clove 

 oil, tease again, and mount in balsam. Note (h. p.) the very small 

 deeply stained fibrils. 



5. Pull out another bundle of tendons : place them in '2 p.c. 

 nitrate of silver for 5 to 10 minutes. Remove to water, move 

 them to and fro once or twice, and gently separate them, renew 

 the water and expose to light, now and then turning them over. 

 In about half-an-hour pass a tendon through alcohols to clove 

 oil. Place on a slide and examine with a low power. The layer 

 of epithelioid cells covering the tendon, will in places be seen 

 (some are often rubbed off in pulling out the tendon from the 

 tail). Cut out the successful pieces, mount in balsam, and examine 

 with a high power. The junctions of the cells are deep black 

 owing to the reduction of silver by the cement substance between 

 them. 



6. Transverse sections of a rather large tendon 

 such as that of the digastric muscle of a cat or rabbit. 

 Stain and mount in balsam. Note the connective 

 tissue sheath around the tendon, the septa from the 

 sheath dividing the tendon into bundles, the branching 

 tendon-cells between the fibres of the tendon bundles. 



7. Branched connective tissue cells of cor- 

 nea. Take, a pithed frog, cut away the nictitating 

 membrane, squeeze the side of the head to make the 

 eye bulge out, then slice boldly at the edge of the 

 cornea; take up its edge with forceps, and with fine 

 scissors cut through the attached part at its junction 

 with the sclerotic ; any blood which may be on the 

 cornea should be removed by placing it in a watch- 

 glass containing normal saline solution and very gently 

 brushing it. Put it in gold chloride '5 p.c. solution, 



