X] CONNECTIVE TISSUE 73 



gently extend it into its normal shape with two finely 

 pointed glass rods, leave it for thirty to forty minutes, 

 wash well with water, transfer to about 20 c.c. of water 

 just acidulated with acetic acid, and expose to light. 

 When the cornea has become of a red- or blue-violet 

 colour 1 , put it on a slide with glycerine, and carefully 

 scrape both surfaces to remove the epithelium, examine 

 it under a low power to see that the epithelium is 

 removed, when it is, mount in formic glycerine and 

 examine it under a high power. If the cornea does 

 not become properly stained in a day, place it in a test- 

 tube with a saturated or nearly saturated solution of 

 tartaric acid, and heat for a few minutes to a tempera- 

 ture at which the test-tube can barely be held in the 

 palm of the hand. This will probably complete the 

 reduction. 



Note the stained connective tissue corpuscles, with 

 numerous fine branching processes which anastomose 

 with the similar processes of neighbouring cells. This 

 preparation should be preserved for examination of the 

 nerves of the cornea (Lesson xiv. 11). 



8. Moist film of areolar tissue. Cut off the 

 hairs from a patch of the skin of the rat used in 3 ; 

 wipe off loose hairs with a moist cloth, cut through the 

 skin and turn it back. With fine forceps pull up a 

 small piece of sub-cutaneous tissue, cut it off with 

 scissors, put it on a cover-slip; spread it out with 

 needles, continuing to do this until the edges stick to 

 the glass and it is fully extended. Put a small drop 



1 Probably it will not be stained until the following day. 



