76 PRACTICAL HISTOLOGY [x 



to remove adhering blood, pour over it 95 p.c. alcohol and leave 

 for half-an-hour. Then cut out the connective tissue and stain 

 it with haematoxylin and eosin. 



The connective tissue fibres will be stained with eosin, the 

 nuclei of the cells with hsematoxylin. 



A similar preparation may be made of the connective tissue 

 between the muscles of the rat or rabbit. In this case, the piece 

 of tissue between the muscles may be picked up with forceps, 

 cut out, and spread out to make a moist film in the manner 

 given in 8. When the edges are dry, the tissue is fixed with 

 alcohol. 



13. Make a moist film preparation of the subcutaneous 

 tissue of a foetal or new-born mammal 1 . 



Then (a) fix with alcohol and stain with hoematoxylin and 

 eosin, pass through alcohols and clove oil and mount in balsam, 

 or (6) add a drop or two of 1 p.c. aqueous solution of Spiller's 

 purple or methylene blue, leave for a minute or two, wash with 

 water, and mount in water. 



Note the cells of various shapes, for the most part with faint 

 outlines, but with distinct and rather large nuclei. 



This may also serve to show the development of fat- cells. 



14. Note in the section of costal cartilage prepared 

 in Lesson ix. 6 the connective tissue layer outside and 

 closely attached to it forming the perichondrium ; in 

 places sections of tendons running into the cartilage 

 will probably be seen. 



15. Pigment-cells. Pin out on a frog-board one 

 of the webs between the toes of the frog used in 7, 

 and observe first under a low and then under a high 

 power. There will be seen large corpuscles loaded 

 with dark pigment, and possessing numerous branched 



1 An animal just killed serves best, but one preserved in picric 

 acid or Mliller's fluid will answer the purpose. 



