80 PRACTICAL HISTOLOGY [x 



Fat tissue for sections may be taken either from the omentum, 

 or from the mass at the hilus of the kidney. 



Quinoline blue dissolved in dilute alcohol is sometimes used 

 for staining fat. The sections are overstained, and decolourized 

 by 35 p.c. potassium hydrate. Sudan III in alcoholic solution is 

 a better stain, the sections are mounted in glycerine. 



Sections containing fat which have been treated with osmic 

 acid, are best treated with chloroform and chloroform balsam 

 instead of xylol and xylol balsam, since xylol has a more or less 

 solvent action on the fat. 



For action of osmic acid on fat cp. App. p. 325. 



Elastic tissues are best stained with orcein (cp. p. 312), but 

 good preparations are also obtained by hardening in chromic 

 acid, and staining in saffranin. 



White fibrous tissue is rapidly and deeply stained with acid 

 magenta (acid fuchsin). See Appendix p. 311 for stain with 

 aniline blue. 



For removal of pigment cp. App. p. 327. 



