LESSON XII. 



CILIATED CELLS. UNSTRIATED AND 

 CARDIAC MUSCLES. 



1. Ciliated Cells. Cut off the head of a recently 

 pithed frog, and cut away the lower jaw. With fine 

 forceps and scissors, cut out on one side a piece of the 

 mucous membrane of the roof of the mouth and place 

 this on a slide, mucous surface uppermost. Pour 2 or 3 

 drops of '5 p.c. osmic acid on the remainder of the 

 mucous membrane of the roof, cover it with a watch- 

 glass for 15 to 20 min. and continue as in 6. 



a. To the piece of membrane on the slide add a 

 drop of '6 p.c. sodium chloride ; scrape the epithelium 

 from a portion of the membrane and tease some of the 

 scrapings in the salt solution. Place a fragment of paper 

 at the edge of the drop ; and cover. Note 



The shimmering appearance caused by the move- 

 ments of the cilia in the larger portions. 



The movements of the cilia in the isolated cells or 

 clumps of cells. Observe carefully the cilia which are 

 moving slowly ; it may be seen that the down stroke 

 (contraction) takes place more quickly than the return 



