94 PRACTICAL HISTOLOGY [XII 



of dilute glycerine to the edge of the cover-slip so that 

 it gradually takes the place of the water, but the cilia 

 will then be less obvious. 



2. Unstriated muscle. Remove the stomach of 

 the frog used in 1. Cut it open, and pin it out. Take 

 up the edge of the mucous membrane with forceps, 

 partly tear and partly cut it away from the muscular 

 coat. Place a piece of the muscular coat about 3 mm. 

 square in a watch-glass with 35 p.c. potassium hydrate. 

 In 20 to 30 minutes, tease the piece in the watch-glass 

 into smallish fragments, remove one or two of these to 

 a slide, tease further, cover and tap lightly. Note 



(h. P .) 



The isolated muscle cells, long cells tapering at 

 the ends ; in some the nucleus here pale and watery 

 looking will be seen in the mid and broader region. 



The tapering ends of the cells projecting from the 

 less teased fragments of tissue. 



3. Cut out one half of the bladder of a pithed 

 frog ; put it in normal salt solution and cut it open. 

 Make a moist film preparation, mucous membrane 

 uppermost (Less. x. 8), rubbing it gently with the 

 finger or with a brush to remove the epithelium and 

 to extend the tissue as much as possible. Fix with 

 alcohol. Examine (h. p.) to see that the epithelium is 

 removed. Press with blotting-paper, add hsematoxylin 

 at once. When it is rather deeply stained, wash, blot 

 again, and add eosin in 75 p.c. alcohol. In about a 

 minute, pour off the eosin, cover with 95 p.c. alcohol 

 until the red colour is not very marked ; blot, a b once 

 add clove oil. Mount in balsam. 



