122 PRACTICAL HISTOLOGY [XV 



DEMONSTRATIONS. 



1. Longitudinal section of spinal ganglion, to show 

 the anterior and posterior roots attached to it, the 

 anterior root running past the nerve cells and joining 

 the fibres of the posterior root at or near the lower end 

 of the ganglion. 



2. Section to show basophil granules and masses 

 of sympathetic ganglion cells (cp. 3). 



3. Section of spinal cord of mammalian embryo or 

 of chick prepared by Golgi method (cp. p. 240), show- 

 ing multipolar cells with a single axon, and many 

 dendrons. 



4. Section of spinal ganglion of chick prepared by 

 Golgi method, showing unipolar cells with dividing 

 process. 



5. Section of spinal ganglion with blood vessels 

 injected. 



NOTES. 



Isolation of Cells in Ganglia. The bipolar spinal ganglion 

 cells of the skate are easily isolated, cp. text 7. A ganglion 

 may also be placed in -5 p.c. osmic acid for half-an-hour and 

 teased (with or without staining) in dilute glycerine. 



The spinal ganglion cells of higher vertebrates, and sym- 

 pathetic ganglion cells are not easily isolated. The following 

 method is perhaps the best. Place the ganglion in '5 to 1 p.c. 



