220 PRACTICAL HISTOLOGY [XXVIII 



Nose of newt. The upper jaw of a pithed newt is cut off in 

 front of the eyes, the anterior part is cut away, the cut passing a 

 little behind the nostrils. The nasal portion is hardened in 

 Flemming's fluid, decalcified, stained in bulk with picrocarmine 

 or with alum carmine, cut in paraffin. A similar preparation 

 showing all the nasal chambers may be made in any small 

 mammal. 



The isolation method given in B 2 serves also for mammals ; 

 the olfactory mucous membrane may be placed for an hour in 

 33 p.c. alcohol before being placed in the osmic acid. 



The nerve endings in the taste cells and the sensory nerve 

 endings in the olfactory mucous membrane may be brought out 

 by the methylene-blue injection method (20 to 30 minutes after 

 injection, sections of a papilla foliata may be cut free-hand, and 

 exposed to the air in a drop of salt solution). For the olfactory 

 cells the Golgi method is used (cp. p. 240). 





